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Then, cells were trypsinized and 2 10 6 cells incubated with 0.5 mmol L 1 of the spin probe CMH (1-hydroxy-3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine), which detects intracellular and extracellular superoxide ion, peroxyl radical, peroxynitrite, and nitrogen dioxide 28