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Peptides derived from cytoskeletal proteins also tended to increase in abundance with age, including proteoforms of dematin actin-binding protein (DMTN), paralemmin-1 (PALM) and microtubule-associated proteins 1/2 (MAP1/2), which may represent degradation of full-length cytoskeletal proteins in later life, leading to cytoskeletal instability 36

Surface plasmon resonance (SPR) spectroscopy The kinetics of the binding of the FBXO22 protein to either full-length BPC157 or single-site alanine-mutated BPC157 were assessed via an SPR assay on a Biacore T200 SPR system (Cytiva)

Osteoclast inhibition

SOFG staining revealed that the NP exhibited abundant proteoglycan content, and no significant disc degeneration was observed (Fig

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