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Additionally, ferroptosis-induced tubular injury promotes EMT via FTH downregulation, releasing free iron that further amplifies ferroptosis and converts epithelial cells to pro-fibrotic mesenchymal cells

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the membranes were then washed several times with water and TBS (PBS, 0.05% Tween-20), a 1 :5000 dilution of horseradish-peroxidase conjugated goat anti-rabbit antiserum was added, and the membranes further incubated for 1 h at room temperature

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Microcytosis may be iron-independent and be related to direct mTOR inhibition affecting protein translation, cell growth, and cell cycle progression [31, 32]

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